PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.

196 – 210 of 3,795 results

196

Invitrogen™ dATP (100 mM)

Invitrogen dATP is prepared to a final concentration of 100 mM in highly purified water. 100 mM dATP is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Pricing & Availability

197

Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)

Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	Medium
Fidelity (vs. Taq)	1X
Format	Tube
Size (Final Product)	5 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	Hot Start PCR Master Mix
Reaction Speed	Standard
Hot Start	Built-In Hot Start
Overhang	3'-A
Concentration	2X
Starting Material	DNA
For Use With (Application)	Hot-start PCR

198

Invitrogen™ AccuPrime™ Taq DNA Polymerase System

The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	2X
Overhang	3'-A
Concentration	10X
For Use With (Application)	Hot-start PCR
PCR Method	1-step RT-qPCR,Multiplex qPCR
Detection Method	Primer-probe
Polymerase	AccuPrime Taq DNA Polymerase
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase System
Reaction Speed	Standard
Hot Start	Built-In Hot Start

199

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Applied Biosystems™ TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

TaqMan™ Fast Universal PCR Master Mix contains all of the components, excluding the template and primers, for superior real-time qPCR.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Concentration	2X
For Use With (Application)	Gene Expression,Genotyping,miRNA analysis
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq DNA Polymerase
Format	Tube
Product Type	Fast Universal PCR Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type	DNA (Genomic), cDNA
Reaction Speed	Fast
Product Line	AmpErase, TaqMan

200

Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit

The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng).

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Platinum SuperFi U DNA Polymerase
GC-Rich PCR Performance	High
Format	Kit
Reverse Transcriptase	SuperScript IV
Size (Final Product)	Up to 10 kb
Final Product Type	PCR Amplified cDNA
Reaction Speed	30 min.
Reagent Type	Reverse Transcription
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription, Preamplification
Starting Material	Cells or RNA
For Use With (Application)	Next-Generation Sequencing (NGS),Real Time PCR (qPCR)

201

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Invitrogen™ Platinum™ Taq DNA Polymerase

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.

Pricing & Availability
Specifications

Polymerase	Platinum Taq DNA Polymerase
GC-Rich PCR Performance	Low
Fidelity (vs. Taq)	1X
Format	Tube
Size (Final Product)	5 kb or less
Reaction Format	Separate Components
Product Type	DNA Polymerase
Hot Start	Built-In Hot Start
Overhang	3'-A
Starting Material	DNA
For Use With (Application)	Hot-start PCR
Exonuclease Activity	5' - 3'

202

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Invitrogen™ SuperScript™ IV First-Strand Synthesis System

The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Format	Kit
Reverse Transcriptase	SuperScript IV
Size (Final Product)	Up to 12.3 kb
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	10 min.
Reagent Type	Reverse Transcription
Quantity	Each
Optimal Reaction Temperature	50°C
Technique	Reverse Transcription
Starting Material	RNA

203

Invitrogen™ AccuPrime™ Pfx SuperMix

AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.

Pricing & Availability

204

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Applied Biosystems™ TaqMan™ Genotyping Master Mix

Optimized for end-point fluorescence detection in SNP genotyping applications.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2 X
Purity or Quality Grade	UP (Ultra Pure)
Concentration	2X
For Use With (Application)	Genotyping
PCR Method	qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Genotyping Target	Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known)
Product Type	Genotyping Master Mix
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7
Sample Type	DNA (Genomic)
Reaction Speed	Standard
Technique	SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation)
Product Line	TaqMan

205

Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Format	Kit
Reverse Transcriptase	MultiScribe
Size (Final Product)	Up to 7 kb
Reaction Format	Premixed Components
Final Product Type	First-Strand cDNA
Reaction Speed	30 to 60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Yes
Optimal Reaction Temperature	37°C
Technique	Reverse Transcription
Starting Material	RNA
For Use With (Application)	Real Time PCR (qPCR),RT-PCR

206

Applied Biosystems™ VetMAX™-Plus One-Step RT-PCR Kit

For use with VetMAX™ Reagents and VetMAX™ Controls.

Pricing & Availability

207

Thermo Scientific™ Phusion Blood Direct PCR Kit

Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Pricing & Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Phusion Blood II DNA Polymerase
GC-Rich PCR Performance	High
Reaction Format	Separate Components
Product Type	Direct PCR Kit
Sample Type	Blood
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2 U/μL
Starting Material	DNA
PCR Method	Direct PCR

208

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Applied Biosystems™ TaqMan™ MicroRNA Reverse Transcription Kit

Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	10 X
Reverse Transcriptase	MultiScribe™
Final Product Type	cDNA
Concentration	10X
For Use With (Application)	miRNA analysis
PCR Method	2-step RT-qPCR
Detection Method	Primer-probe
Size (Final Product)	200 bp or less
Reaction Format	Separate Components
Product Type	MicroRNA Reverse Transcription Kit
Sample Type	RNA
Reaction Speed	Fast
Optimal Reaction Temperature	42°C
Product Line	TaqMan

209

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Applied Biosystems™ Human B2M (Beta-2-Microglobulin) Endogenous Control (VIC™/MGB probe, primer limited)

Human B2M (Beta-2-Microglobulin) Endogenous Control (VIC™/MGB probe, primer limited)

Pricing & Availability

210

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Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)

Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)

Pricing & Availability

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Applied Biosystems™ Human B2M (Beta-2-Microglobulin) Endogenous Control (VIC™/MGB probe, primer limited) Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited) Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Applied Biosystems™ Human B2M (Beta-2-Microglobulin) Endogenous Control (VIC™/MGB probe, primer limited)

Applied Biosystems™ Human GAPD (GAPDH) Endogenous Control (VIC™/MGB probe, primer limited)